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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | enhancer of mRNA decapping 3 |
| Gene Symbol: | EDC3 |
| Synonyms: | YJDC; LSM16; MRT50; YJEFN2; hYjeF_N2-15q23; EDC3 |
| Gene ID: | 80153 |
| UniProt ID: | Q96F86 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 159-508 of human EDC3 (NP_079359.2). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.23 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-EDC3 antibody |
Background
This gene encodes a protein that is important in mRNA degradation. The encoded protein is a component of a decapping complex that promotes efficient removal of the monomethylguanosine (m7G) cap from mRNAs, as part of the 5' to 3' mRNA decay pathway. Mutations in this gene have been identified in human patients with an autosomal recessive form of intellectual disability.
Images
 | Western blot analysis of various lysates using EDC3 Rabbit pAb (A13763) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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