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| Reactivity: | Human, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | eukaryotic translation initiation factor 2 alpha kinase 1 |
| Gene Symbol: | EIF2AK1 |
| Synonyms: | HCR; HRI; hHRI; LEMSPAD; EIF2AK1 |
| Gene ID: | 27102 |
| UniProt ID: | Q9BQI3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 337-586 of human EIF2AK1 (NP_055228.2). |
| Dilution: | WB 1:500-1:2000; IHC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 1.51 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-EIF2AK1 antibody |
Background
The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from K-562 cells, using EIF2AK1 Rabbit pAb (A14119) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Western blot analysis of lysates from Rat liver, using EIF2AK1 Rabbit pAb (A14119) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver using EIF2AK1 Rabbit pAb (A14119) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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