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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | eukaryotic translation initiation factor 4E binding protein 1 |
| Gene Symbol: | EIF4EBP1 |
| Synonyms: | BP-1; 4EBP1; 4E-BP1; PHAS-I; eIF4EBP1 |
| Gene ID: | 1978 |
| UniProt ID: | Q13541 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-118 of human eIF4EBP1 (NP_004086.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200; IF/IC 1:100-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.45 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-EIF4EBP1 antibody |
Background
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Images
 | Western blot analysis of various lysates using eIF4EBP1 Rabbit pAb (A1248) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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