Emerin/EMD Rabbit pAb (20 μl)

Emerin/EMD Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A13925
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse    
Applications: WB, IHC, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: emerin
Gene Symbol: EMD
Synonyms: STA; EDMD; LEMD5; Emerin/EMD
Gene ID: 2010
UniProt ID: P50402
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Emerin/Emerin/EMD (NP_000108.1).
Dilution: WB 1:500-1:2000; IHC 1:50-1:200; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 2.03 mg/ml
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-EMD antibody




Background

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.




Images

ATP4B antibodyWestern blot analysis of various lysates using Emerin/EMD Rabbit pAb (A13925) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Emerin/EMD Rabbit pAb (A13925) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human uterine cancer using Emerin/EMD Rabbit pAb (A13925) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of U-2 OS cells using Emerin/EMD Rabbit pAb (A13925) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2OS cells using Emerin/EMD Rabbit pAb (A13925) at dilution of 1:100. Blue: DAPI for nuclear staining.



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