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| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | EME1 rabbit polyclonal |
Gene Name: | Crossover junction endonuclease EME1 |
Synonyms: | EME1, hMMS4, MMS4, MMS4 homolog, MMS4L, Essential Meiotic Endonuclease 1 Homolog 1, homolog of yeast EME1 endonuclease |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse EME1 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 63 kDa |
Observed MW. | 63 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 500-1: 1000 | HeLa, Jurkat, 293T, K562, SW480 |
Background
Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.
Images
 | Western blot analysis of EME1 (GB113236) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |