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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | enolase 1 |
| Gene Symbol: | ENO1 |
| Synonyms: | NNE; PPH; MPB1; ENO1L1; ENO1-IT1; HEL-S-17; ENO1 |
| Gene ID: | 2023 |
| UniProt ID: | P06733 |
| Clone ID: | 9E5R2 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ENO1 (P06733). |
| Dilution: | WB 1:1000-1:6000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.37 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-ENO1 antibody |
Background
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
Images
 | Western blot analysis of various lysates using ENO1 Rabbit mAb (A11448) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
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