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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Estrogen receptor 1 |
| Gene Symbol: | ESR1 |
| Synonyms: | ER; ESR; Era; ESRA; ESTRR; NR3A1; ESRα |
| Gene ID: | 2099 |
| UniProt ID: | P03372 |
| Clone ID: | 4L8K5 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Estrogen Receptor alpha (ESRα) (P03372). |
| Dilution: | WB 1:1000-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.7 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-ESR1 antibody |
Background
This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.
Images
 | Western blot analysis of lysates from MCF7 cells, using ESRα Rabbit mAb (A12976) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
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