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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC,IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | estrogen related receptor alpha |
| Gene Symbol: | ESRRA |
| Synonyms: | ERR1; ERRa; ESRL1; NR3B1; ERRalpha; ESRRA |
| Gene ID: | 2101 |
| UniProt ID: | P11474 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-76 of human ESRRA (NP_004442.3). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:100; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 3 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-ESRRA antibody |
Background
The protein encoded by this gene is a nuclear receptor that is most closely related to the estrogen receptor. This protein acts as a site-specific transcription factor and interacts with members of the PGC-1 family of transcription cofactors to regulate the expression of most genes involved in cellular energy production as well as in the process of mitochondrial biogenesis. A processed pseudogene of ESRRA is located on chromosome 13q12.1.
Images
 | Western blot analysis of various lysates using ESRRA Rabbit pAb (A14184) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry analysis of paraffin-embedded Human uterus using ESRRA Rabbit pAb (A14184) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain using ESRRA Rabbit pAb (A14184) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain using ESRRA Rabbit pAb (A14184) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of paraffin-embedded Rat colon using ESRRA Rabbit pAb (A19069) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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