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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | enhancer of zeste 1 polycomb repressive complex 2 subunit |
| Gene Symbol: | EZH1 |
| Synonyms: | KMT6B; EZH1 |
| Gene ID: | 2145 |
| UniProt ID: | Q92800 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 160-270 of EZH1 (NP_001982.2). |
| Dilution: | WB 1:200-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 3.03 mg/mL |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-EZH1 antibody |
Background
EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).
Images
 | Western blot analysis of lysates from RAW264.7 cells, using EZH1 Rabbit pAb (A15272) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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