Coagulation factor III/Tissue Factor Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A1378
Availability: In Stock

$108.00

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Description

Reactivity:	Human, Mouse, Rat
Applications:	WB, IF/IC, ELISA
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Polyclonal antibody
Gene Name:	coagulation factor III, tissue factor
Gene Symbol:	F3
Synonyms:	TF; TFA; CD142; Coagulation factor III/Tissue Factor
Gene ID:	2152
UniProt ID:	P13726
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-251 of human Coagulation factor III/Tissue Factor (NP_001984.1).
Dilution:	WB 1:500-1:1000; IF/IC 1:50-1:200
Purification Method:	Affinity purification
Concentration:	1.29 mg/ml
Buffer:	PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze/thaw cycles.
Documents:	Manual-F3 antibody

Background

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces, for example, on monocytes. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. Platelets and monocytes have been shown to express this coagulation factor under procoagulatory and proinflammatory stimuli, and a major role in HIV-associated coagulopathy has been described. Platelet-dependent monocyte expression of coagulation factor III has been described to be associated with Coronavirus Disease 2019 (COVID-19) severity and mortality. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.

Images

	Western blot analysis of lysates from BxPC-3 cells, using Coagulation factor III/Tissue Factor Rabbit pAb (A1378) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of various lysates using Coagulation factor III/Tissue Factor Rabbit pAb (A1378) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A431 cells using Coagulation factor III/Tissue Factor Rabbit pAb (A1378) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.