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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | Fc gamma receptor IIa |
| Gene Symbol: | FCGR2A |
| Synonyms: | CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1; FcgammaRIIa; FCGR2A |
| Gene ID: | 2212 |
| UniProt ID: | P12318 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 34-316 of human FCGR2A (NP_001129691.1). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.26 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-FCGR2A antibody |
Background
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of various lysates using FCGR2A Rabbit pAb (A1388) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of paraffin-embedded mouse spleen using FCGR2A Rabbit pAb (A1388) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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