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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | fms related receptor tyrosine kinase 3 |
| Gene Symbol: | FLT3 |
| Synonyms: | FLK2; STK1; CD135; FLK-2; FLT3 |
| Gene ID: | 2322 |
| UniProt ID: | P36888 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 701-800 of human FLT3 (NP_004110.2). |
| Dilution: | WB 1:100-1:500; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.4 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-FLT3 antibody |
Background
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Images
 | Western blot analysis of various lysates, using FLT3 Rabbit pAb (A12437) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat pancreas using FLT3 Rabbit pAb (A12437) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver cancer using FLT3 Rabbit pAb (A12437) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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