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| Reactivity: | Human, Rat |
| Applications: | WB,IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | frizzled class receptor 5 |
| Gene Symbol: | FZD5 |
| Synonyms: | HFZ5; C2orf31; FZD5 |
| Gene ID: | 7855 |
| UniProt ID: | Q13467 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 130-240 of human FZD5 (NP_003459.2). |
| Dilution: | WB 1:500-1:2000; IHC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 0.09 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-FZD5 antibody |
Background
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand.
Images
 | Western blot analysis of lysates from rat kidney, using FZD5 Rabbit pAb (A12775) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon using FZD5 Rabbit pAb (A12775) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat kidney using FZD5 Rabbit pAb (A12775) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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