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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | G3BP stress granule assembly factor 1 |
| Gene Symbol: | G3BP1 |
| Synonyms: | G3BP; HDH-VIII; G3BP1 |
| Gene ID: | 10146 |
| UniProt ID: | Q13283 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 131-330 of human G3BP1 (NP_938405.1). |
| Dilution: | WB 1:100-1:500; IHC 1:50-1:100; IF/IC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 1.75 mg/mL |
| Buffer: | Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-G3BP1 antibody |
Background
This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Images
 | Western blot analysis of lysates from 293T cells, using G3BP1 Rabbit pAb (A14836) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon tissue using G3BP1 Rabbit pAb (A14836) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using G3BP1 Rabbit pAb (A14836) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using G3BP1 Rabbit pAb (A14836) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using G3BP1 Rabbit pAb (A14836) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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