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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | glycoprotein nmb |
| Gene Symbol: | GPNMB |
| Synonyms: | NMB; HGFIN; PLCA3; GPNMB |
| Gene ID: | 10457 |
| UniProt ID: | Q14956 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-486 of human GPNMB (NP_001005340.1). |
| Dilution: | WB 1:1000-1:5000 |
| Purification Method: | Affinity purification |
| Concentration: | 2.25 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-GPNMB antibody |
Background
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from SK-MEL-28 cells, using GPNMB Rabbit pAb (A14270) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of lysates from Rat eye, using GPNMB Rabbit pAb (A14270) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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