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| Reactivity: | Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | glutamate ionotropic receptor NMDA type subunit 1 |
| Gene Symbol: | GRIN1 |
| Synonyms: | NR1; MRD8; GluN1; NMDA1; DEE101; NDHMSD; NDHMSR; NMD-R1; NMDAR1 |
| Gene ID: | 2902 |
| UniProt ID: | Q05586 |
| Clone ID: | 1H1T3 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 800-900 of human NMDAR1 (Q05586). |
| Dilution: | WB 1:1000-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.8 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-GRIN1 antibody |
Background
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.
Images
 | Western blot analysis of lysates from Mouse brain, using NMDAR1 Rabbit mAb (A11699) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Confocal imaging of Mouse brain using NMDAR1 Rabbit mAb (A11699,dilution 1:100)(Red). DAPI was used for nuclear staining (blue). Objective: 60x. |
 | Immunofluorescence analysis of paraffin-embedded Mouse brain tissue using NMDAR1 Rabbit mAb (A11699) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining. |
 | Immunofluorescence analysis of paraffin-embedded Rat brain tissue using NMDAR1 Rabbit mAb (A11699) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining. |
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