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| Reactivity: | Human, Mouse |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | general transcription factor IIIC subunit 6 |
| Gene Symbol: | GTF3C6 |
| Synonyms: | C6orf51; TFIIIC35; bA397G5.3; GTF3C6 |
| Gene ID: | 112495 |
| UniProt ID: | Q969F1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human GTF3C6 (NP_612417.1). |
| Dilution: | WB 1:2000-1:6000 |
| Purification Method: | Affinity purification |
| Concentration: | 8.28 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-GTF3C6 antibody |
Background
RNA polymerases are unable to initiate RNA synthesis in the absence of additional proteins called general transcription factors (GTFs). GTFs assemble in a complex on the DNA promoter and recruit the RNA polymerase. GTF3C family proteins (e.g., GTF3C1, MIM 603246) are essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA (MIM 180420), tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin.
Images
 | Western blot analysis of various lysates, using GTF3C6 Rabbit pAb (A13233) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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