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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA, ChIP |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | H2A.X variant histone |
| Gene Symbol: | H2AX |
| Synonyms: | H2A.X; H2A/X; H2AFX; Histone H2AX |
| Gene ID: | 3014 |
| UniProt ID: | P16104 |
| Clone ID: | 4Z3S8 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2AX (P16104). |
| Dilution: | WB 1:1000-1:6000; IHC 1:1000-1:4000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.2 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-H2AX antibody |
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Images
 | Western blot analysis of various lysates using Histone H2AX Rabbit mAb (A11412) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Histone H2AX Rabbit mAb (A11412) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Histone H2AX Rabbit mAb (A11412) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Histone H2AX Rabbit mAb (A11412) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using Histone H2AX Rabbit mAb (A11412) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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