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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | heparin binding growth factor |
| Gene Symbol: | HDGF |
| Synonyms: | HMG1L2; HDGF |
| Gene ID: | 3068 |
| UniProt ID: | P51858 |
| Clone ID: | 5U7A7 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HDGF (P51858). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.33 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-HDGF antibody |
Background
This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Images
 | Western blot analysis of various lysates, using HDGF Rabbit mAb (A0589) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of various lysates, using HDGF Rabbit mAb (A0589) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Confocal imaging of paraffin-embedded Human colon tissue using HDGF Rabbit mAb (A0589, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining. |
 | Confocal imaging of paraffin-embedded Mouse colon tissue using HDGF Rabbit mAb (A0589, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining. |
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