Your shopping cart is empty!
| Reactivity: | Human, Mouse |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | histamine N-methyltransferase |
| Gene Symbol: | HNMT |
| Synonyms: | HMT; MRT51; HNMT-S1; HNMT-S2; HNMT |
| Gene ID: | 3176 |
| UniProt ID: | P50135 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.00 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-HNMT antibody |
Background
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.
Images
 | Western blot analysis of various lysates using HNMT Rabbit pAb (A13499) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunoprecipitation analysis of 200 μg extracts of HT-29 cells using 1 μg HNMT antibody (A13499). Western blot was performed from the immunoprecipitate using HNMT antibody (A13499) at a dilution of 1:1000. |
You may also be interested in:
Related Products
-10%
-16%
