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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgM,Kappa |
| Clonality: | Polyclonal antibody |
| Gene Name: | heat shock transcription factor 1 |
| Gene Symbol: | HSF1 |
| Synonyms: | HSTF1; HSF1 |
| Gene ID: | 3297 |
| UniProt ID: | Q00613 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 350-529 of human HSF1 (NP_005517.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:100; IF/IC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 0.83 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-HSF1 antibody |
Background
The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.
Images
 | Western blot analysis of various lysates using HSF1 Rabbit pAb (A13765) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Rat spleen using HSF1 Rabbit pAb (A13765) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of C6 cells using HSF1 Rabbit pAb (A13765) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using HSF1 Rabbit pAb (A13765) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using HSF1 Rabbit pAb (A13765) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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