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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IP, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | HtrA serine peptidase 2 |
| Gene Symbol: | HTRA2 |
| Synonyms: | OMI; MGCA8; PARK13; PRSS25; HTRA2 |
| Gene ID: | 27429 |
| UniProt ID: | O43464 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 334-458 of human HTRA2 (NP_037379.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.15 mg/mL |
| Buffer: | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-HTRA2 antibody |
Background
This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
Images
 | Western blot analysis of lysates from MCF7 cells, using HTRA2 Rabbit pAb (A14877) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunoprecipitation analysis of 200 μg extracts of MCF-7 cells, using 3 μg HTRA2 antibody (A14877). Western blot was performed from the immunoprecipitate using HTRA2 antibody (A14877) at a dilution of 1:1000. |
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