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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | interferon gamma |
| Gene Symbol: | IFNG |
| Synonyms: | IFG; IFI; IMD69; IFN gamma |
| Gene ID: | 3458 |
| UniProt ID: | P01579 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-161 of IFN gamma (NP_997468.1). |
| Dilution: | WB 1:2000-1:10000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.77mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-IFNG antibody |
Background
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
Images
 | Western blot analysis of Recombinant Human IFN-gamma Protein (RP01038), using IFN gamma Rabbit pAb (A12450) at 1:9000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 1ng/0.5ng/0.25ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung using IFN gamma Rabbit pAb (A12450) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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