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| Reactivity: | Human |
| Applications: | WB, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | interleukin 18 |
| Gene Symbol: | IL18 |
| Synonyms: | IGIF; IL-18; IL-1g; IL1F4; IL18 |
| Gene ID: | 3606 |
| UniProt ID: | Q14116 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 37-193 of human IL18 (NP_001553.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.52mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-IL18 antibody |
Background
The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of Recombinant Human IL-18 Protein (RP00006) using IL18 Rabbit pAb (A1115) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 10 ng/1 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg IL18 antibody (A1115). Western blot was performed from the immunoprecipitate using IL18 antibody (A1115) at a dilution of 1:1000. |
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