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| Reactivity: | Human, Mouse |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | ISY1 splicing factor homolog |
| Gene Symbol: | ISY1 |
| Synonyms: | FSAP33; ISY1 |
| Gene ID: | 57461 |
| UniProt ID: | Q9ULR0 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-307 of human ISY1 (NP_001186398.1). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 1.28 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-ISY1 antibody |
Background
Enables RNA binding activity. Involved in mRNA splicing, via spliceosome. Located in nucleus. Part of U2-type catalytic step 1 spliceosome and catalytic step 2 spliceosome.
Images
 | Western blot analysis of various lysates using ISY1 Rabbit pAb (A13828) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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