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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | cytidine deaminase |
| Gene Symbol: | CDA |
| Synonyms: | CDD |
| Gene ID: | 978 |
| UniProt ID: | P32320 |
| Clone ID: | 24GB6890 |
| Immunogen: | Recombinant protein of human CDA |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene CDA encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias.
Images
 | Western blotting analysis using anti-cytidine deaminase antibody (Cat#63896). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cytidine deaminase antibody (Cat#63896, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-cytidine deaminase antibody (Cat#63896). Cytidine deaminase expression in wild type (WT) and cytidine deaminase (CDA) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cytidine deaminase antibody (Cat#63896, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Cytidine deaminase expression in HepG2 cells using anti-Cytidine deaminase antibody (Cat#63896, 1:2,000). Green, isotype control; red, Cytidine deaminase. |
 | Immunocytochemical staining of HepG2 cells with anti-Cytidine deaminase antibody (Cat#63896, 1:1,000). Nuclei were stained blue with DAPI; Cytidine deaminase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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