Your shopping cart is empty!
| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | acetyl-CoA carboxylase alpha |
| Gene Symbol: | ACACA |
| Synonyms: | ACC; ACAC; ACC1; ACCA; Acac1; hACC1; ACACAD; ACCalpha; ACACalpha |
| Gene ID: | 31 |
| UniProt ID: | Q13085 |
| Clone ID: | 23GB5645 |
| Immunogen: | A synthesized peptide derived from human Acetyl Coenzyme A Carboxylase |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-Acetyl-CoA carboxylase alpha antibody (Cat#62029). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Acetyl-CoA carboxylase alpha antibody (Cat#62029, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Acetyl-CoA carboxylase α antibody (Cat#62029). Acetyl-CoA carboxylase α expression in wild type (WT) and Acetyl-CoA carboxylase α shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Acetyl-CoA carboxylase α antibody (Cat#62029, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Acetyl-CoA carboxylase alpha expression in HepG2 cells using Acetyl-CoA carboxylase alpha antibody (Cat#62029, 1:2,000). Green, isotype control; red, Acetyl-CoA carboxylase alpha. |
 | Immunocytochemical staining of HepG2 cells with anti-Acetyl-CoA carboxylase alpha antibody (Cat#62029, 1:1,000). Nuclei were stained blue with DAPI; Acetyl-CoA carboxylase alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
You may also be interested in:
