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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | annexin A1 |
| Gene Symbol: | ANXA1 |
| Synonyms: | ANX1; LPC1 |
| Gene ID: | 301 |
| UniProt ID: | P04083 |
| Clone ID: | 24GB6230 |
| Immunogen: | Recombinant protein of human ANXA1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The gene ANXA1 encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.
Images
 | Western blotting analysis using anti-annexin A1 antibody (Cat#63500). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A1 antibody (Cat#63500, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-annexin A1 antibody (Cat#63500). Annexin A1 expression in wild type (WT) and annexin A1 (ANXA1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-annexin A1 antibody (Cat#63500, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Annexin A1 expression in H9c2 cells using anti-Annexin A1 antibody (Cat#63500, 1:2,000). Green, isotype control; red, Annexin A1. |
 | Immunocytochemical staining of H9C2 cells with anti-Annexin A1 antibody (Cat#63500, 1:1,000). Nuclei were stained blue with DAPI; Annexin A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm. |
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