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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | Rho GDP dissociation inhibitor alpha |
| Gene Symbol: | ARHGDIA |
| Synonyms: | GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e |
| Gene ID: | 396 |
| UniProt ID: | P52565 |
| Clone ID: | 24GB6235 |
| Immunogen: | Recombinant protein of human ARHGDIA |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene ARHGDIA encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#63501). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#63501, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#63501). Rho GDP dissociation inhibitor alpha expression in wild type (WT) and Rho GDP dissociation inhibitor alpha (ARHGDIA) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#63501, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Rho GDP dissociation inhibitor alpha expression in HepG2 cells using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#63501, 1:2,000). Green, isotype control; red, Rho GDP dissociation inhibitor alpha. |
 | Immunocytochemical staining of HepG2 cells with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#63501, 1:1,000). Nuclei were stained blue with DAPI; Rho GDP dissociation inhibitor alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Scale bar, 20 μm. |
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