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| Reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG2a |
| Clonality: | Monoclonal antibody |
| Gene Name: | branched chain amino acid transaminase 1 |
| Gene Symbol: | BCAT1 |
| Synonyms: | BCT1; PP18; BCATC; ECA39; MECA39; PNAS121 |
| Gene ID: | 586 |
| UniProt ID: | P54687 |
| Clone ID: | 24GB7945 |
| Immunogen: | Recombinant protein of human BCAT1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene BCAT1 encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.
Images
 | Western blotting analysis using anti-BCAT1 antibody (Cat#63802). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCAT1 antibody (Cat#63802, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-BCAT1 antibody (Cat#63802). BCAT1 expression in wild type (WT) and BCAT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BCAT1 antibody (Cat#63802, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of BCAT1 expression in C2C12 cells using anti-BCAT1 antibody (Cat#63802, 1:1,000). Green, isotype control; red, BCAT1. |
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