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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | cyclin B1 |
| Gene Symbol: | CCNB1 |
| Synonyms: | CCNB |
| Gene ID: | 891 |
| UniProt ID: | P14635 |
| Clone ID: | 24GB4305 |
| Immunogen: | Recombinant protein of Mouse Cyclin B1 (UniProt Entry: P24860) |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein CCNB1 encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.
Images
 | Western blotting analysis using anti-cyclin B1 antibody (Cat#63160). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin B1 antibody (Cat#63160, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-cyclin B1 antibody (Cat#63160). Cyclin B1 expression in wild-type (WT) and cyclin B1 (CCNB1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin B1 antibody (Cat#63160, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Cyclin B1 expression in HeLa cells using anti-Cyclin B1 antibody (Cat#63160, 1:2,000). Green, isotype control; red, Cyclin B1. |
 | Immunocytochemical staining of Hela cells with cyclin B1 antibody (Cat#63160, 1:1,000). Nuclei were stained blue with DAPI; Cyclin B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
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