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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | cell division cycle 34, ubiqiutin conjugating enzyme |
| Gene Symbol: | CDC34 |
| Synonyms: | UBC3; UBCH3; UBE2R1; E2-CDC34 |
| Gene ID: | 997 |
| UniProt ID: | P49427 |
| Clone ID: | 25GB3490 |
| Immunogen: | Recombinant protein of human Cdc34 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein CDC34 encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
Images
 | Western blotting analysis using anti-CDC34 antibody (Cat#61736). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC34 antibody (Cat#61736, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-CDC34 antibody (Cat#61736). CDC34 expression in wild-type (WT) and CDC34 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CDC34 antibody (Cat#61736, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of CDC34 expression in HAP-1 cells using anti-CDC34 antibody (Cat#61736, 1:2,000). Green, isotype control; red, CDC34. |
 | Immunocytochemical staining of HAP1 cells with anti-CDC34 antibody (Cat#61736, 1:1,000). Nuclei were stained blue with DAPI; CDC34 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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