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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | cAMP responsive element binding protein 1 |
| Gene Symbol: | CREB1 |
| Synonyms: | CREB; CREB-1 |
| Gene ID: | 1385 |
| UniProt ID: | P16220 |
| Clone ID: | 24GB15780 |
| Immunogen: | Recombinant protein of human CREB1 |
| Dilution: | WB 1:500-1:2,500 FC 1:1,000 IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene CREB1 encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Images
 | Western blotting analysis using anti-cAMP responsive element binding protein 1 antibody (Cat#64881). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cAMP responsive element binding protein 1 antibody (Cat#64881, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-cAMP responsive element binding protein 1 responsive element binding protein 1 antibody (Cat#64881). CAMP responsive element binding protein 1 responsive element binding protein 1 expression in wild-type (WT) and CREB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cAMP responsive element binding protein 1 responsive element binding protein 1 antibody (Cat#64881, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of cAMP responsive element binding protein 1 expression in C2C12 cells using anti-cAMP responsive element binding protein 1 antibody (Cat#64881, 1:1,000). Green, isotype control; red, cAMP responsive element binding protein 1. |
 | Immunocytochemical staining of C2C12 cells with anti-CAMP responsive element binding protein 1 antibody (Cat#64881, 1:1,000). Nuclei were stained blue with DAPI; CAMP responsive element binding protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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