Your shopping cart is empty!
| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Cystathionine gamma-lyase |
| Gene Symbol: | CTH |
| Synonyms: | CGL; CSE |
| Gene ID: | 1491 |
| UniProt ID: | P32929 |
| Clone ID: | 24GB4795 |
| Immunogen: | A synthesized peptide derived from human Cystathionase/CTH |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The gene CTH encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in three transcript variants encoding different isoforms.
Images
 | Western blotting analysis using anti-CTH antibody (Cat#63268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CTH antibody (Cat#63268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-CTH antibody (Cat#63268). CTH expression in wild type (WT) and CTH shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CTH antibody (Cat#63268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of CTH expression in HepG2 cells using anti-CTH antibody (Cat#63268, 1:2,000). Green, isotype control; red, CTH. |
 | Immunocytochemical staining of HepG2 cells with anti-CTH antibody (Cat#63268, 1:1,000). Nuclei were stained blue with DAPI; CTH was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
You may also be interested in:
