KD-Validated EEF2 Rabbit pAb (20 μl)

KD-Validated EEF2 Rabbit pAb (20 μl)

Cat. #: 64990
Availability: In Stock
$149.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Polyclonal antibody    
Gene Name:   eukaryotic translation elongation factor 2
Gene Symbol:   EEF2
Synonyms:   EF2; EF-2; EEF-2; SCA26
Gene ID:   1938
UniProt ID:   P13639
Immunogen:   A synthesized peptide derived from human EEF2
Dilution:   WB 1:500-1:2,500
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

This gene EEF2 encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.




Images

EEF2 antibodyWestern blotting analysis using anti-EEF2 antibody (Cat#64990). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EEF2 antibody (Cat#64990, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-EEF2 antibody (Cat#64990). EEF2 expression in wild-type (WT) and EEF2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EEF2 antibody (Cat#64990, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).



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