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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | eukaryotic translation initiation factor 4E |
| Gene Symbol: | EIF4E |
| Synonyms: | CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1 |
| Gene ID: | 1977 |
| UniProt ID: | P06730 |
| Clone ID: | 25GB3135 |
| Immunogen: | A synthesized peptide derived from human EIF4E |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-eukaryotic translation initiation factor 4E antibody (Cat#61648). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-eukaryotic translation initiation factor 4E antibody (Cat#61648, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-eukaryotic translation initiation factor 4E antibody (Cat#61648). Eukaryotic translation initiation factor 4E expression in wild type (WT) and eukaryotic translation initiation factor 4E (EIF4E) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-eukaryotic translation initiation factor 4E antibody (Cat#61648, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Eukaryotic translation initiation factor 4E expression in HepG2 cells using anti-Eukaryotic translation initiation factor 4E antibody (Cat#61648, 1:2,000). Green, isotype control; red, Eukaryotic translation initiation factor 4E. |
 | Immunocytochemical staining of HepG2 cells with anti-Eukaryotic translation initiation factor 4E antibody (Cat#61648, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation initiation factor 4E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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