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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | enolase 1 |
| Gene Symbol: | ENO1 |
| Synonyms: | NNE; PPH; MPB1; ENO1L1; ENO1-IT1; HEL-S-17 |
| Gene ID: | 2023 |
| UniProt ID: | P06733 |
| Clone ID: | 25GB2820 |
| Immunogen: | A synthesized peptide derived from human ENO1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This ENO1 gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
Images
 | Western blotting analysis using anti-enolase 1 antibody (Cat#61555). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-enolase 1 antibody (Cat#61555, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-enolase 1 antibody (Cat#61555). Enolase 1 expression in wild-type (WT) and enolase 1 (ENO1) knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-enolase 1 antibody (Cat#61555, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Enolase 1 expression in HepG2 cells using anti-Enolase 1 antibody (Cat#61555, 1:2,000). Green, isotype control; red, Enolase 1. |
 | Immunocytochemical staining of HepG2 cells with anti-Enolase 1 antibody (Cat#61555, 1:1,000). Nuclei were stained blue with DAPI; Enolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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