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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | EWS RNA binding protein 1 |
| Gene Symbol: | EWSR1 |
| Synonyms: | EWS; EWS-FLI1; bK984G1.4 |
| Gene ID: | 2130 |
| UniProt ID: | Q01844 |
| Clone ID: | 25GB3500 |
| Immunogen: | A synthesized peptide derived from human EWSR1 |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene EWSR1 encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.
Images
 | Western blotting analysis using anti-EWS RNA binding protein 1 antibody (Cat#61739). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EWS RNA binding protein 1 antibody (Cat#61739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-EWS RNA binding protein 1 antibody (Cat#61739). EWS RNA binding protein 1 expression in wild-type (WT) and EWS RNA binding protein 1 (EWSR1) knockdown (KD) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-EWS RNA binding protein 1 antibody (Cat#61739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of EWS RNA binding protein 1 expression in HepG2 cells using anti-EWS RNA binding protein 1 antibody (Cat#61739, 1:2,000). Green, isotype control; red, EWS RNA binding protein 1. |
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