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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Fli-1 proto-oncogene, ETS transcription factor |
| Gene Symbol: | FLI1 |
| Synonyms: | EWSR2; FLI-1; SIC-1; BDPLT21 |
| Gene ID: | 2313 |
| UniProt ID: | Q01543 |
| Clone ID: | 25GB2875 |
| Immunogen: | A synthesized peptide derived from human FLI1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene FLI1 encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-FLI1 antibody (Cat#61569). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FLI1 antibody (Cat#61569, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-FLI1 antibody (Cat#61569). FLI1 expression in wild-type (WT) and FLI1 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FLI1 antibody (Cat#61569, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of FLI1 expression in HT-1080 cells using anti-FLI1 antibody (Cat#61569, 1:2,000). Green, isotype control; red, FLI1. |
 | Immunocytochemical staining of HT-1080 cells with anti-FLI1 antibody (Cat#61569, 1:1,000). Nuclei were stained blue with DAPI; FLI1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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