Your shopping cart is empty!
| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Forkhead box A1 |
| Gene Symbol: | FOXA1 |
| Synonyms: | HNF3A; TCF3A |
| Gene ID: | 3169 |
| UniProt ID: | P55317 |
| Clone ID: | 23GB1170 |
| Immunogen: | A synthesized peptide derived from human FOXA1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The gene FOXA1 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.
Images
 | Western blotting analysis using anti-Forkhead box A1 antibody (Cat#68581). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Forkhead box A1 antibody (Cat#68581, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-forkhead box A1 antibody (Cat#68581). Forkhead box A1 expression in wild-type (WT) and forkhead box A1 (FOXA1) shRNA knockdown (KD) HepG2 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-forkhead box A1 antibody (Cat#68581, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of FOXA1 expression in HepG2 cells using anti-FOXA1 antibody (Cat#68581, 1:2,000). Green, isotype control; red, FOXA1. |
 | Immunocytochemical staining of HepG2 cells with Forkhead box A1 antibody (Cat#68581, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
You may also be interested in:
