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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | forkhead box O3 |
| Gene Symbol: | FOXO3 |
| Synonyms: | FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2 |
| Gene ID: | 2309 |
| UniProt ID: | O43524 |
| Clone ID: | 25GB2940 |
| Immunogen: | A synthesized peptide derived from human FoxO3a |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
Images
 | Western blotting analysis using anti-forkhead box O3 antibody (Cat#61588). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-forkhead box O3 antibody (Cat#61588, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-forkhead box O3 antibody (Cat#61588). Forkhead box O3 expression in wild-type (WT) and forkhead box O3 (FOXO3) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-forkhead box O3 antibody (Cat#61588, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Forkhead box O3 expression in HepG2 cells using anti-Forkhead box O3 antibody (Cat#61588, 1:2,000). Green, isotype control; red, Forkhead box O3. |
 | Immunocytochemical staining of HepG2 cells with anti-Forkhead box O3 antibody (Cat#61588, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box O3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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