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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | growth factor receptor bound protein 7 |
| Gene Symbol: | GRB7 |
| Synonyms: | GRB7; Growth Factor Receptor Bound Protein 7; Growth Factor Receptor-Bound Protein 7; Epidermal Growth Factor Receptor GRB-7; GRB7 Adapter Protein; B47 |
| Gene ID: | 2886 |
| UniProt ID: | Q14451 |
| Clone ID: | 24GB6000 |
| Immunogen: | A synthesized peptide derived from human GRB7 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-anti-growth factor receptor bound protein 7 antibody (Cat#61153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-growth factor receptor bound protein 7 antibody (Cat#61153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-growth factor receptor bound protein 7 antibody (Cat#61153). Growth factor receptor bound protein 7 expression in wild type (WT) and growth factor receptor bound protein 7 (GRB7) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-growth factor receptor bound protein 7 antibody (Cat#61153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of growth factor receptor bound protein 7 expression in HepG2 cells using growth factor receptor bound protein 7 antibody (Cat#61153, 1:2,000). Green, isotype control; red, growth factor receptor bound protein 7. |
 | Immunocytochemical staining of HepG2 cells with anti-growth factor receptor bound protein 7 antibody (Cat#61153, 1:1,000). Nuclei were stained blue with DAPI; Growth factor receptor bound protein 7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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