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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | protein-coupled receptor kinase 2 |
| Gene Symbol: | GRK2 |
| Synonyms: | BARK1; ADRBK1; BETA-ARK1 |
| Gene ID: | 156 |
| UniProt ID: | P25098 |
| Clone ID: | 23GB1515 |
| Immunogen: | A synthesized peptide derived from human GRK2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The gene GRK2 G encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease.
Images
 | Western blotting analysis using anti-GRK2 antibody (Cat#63642). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GRK2 antibody (Cat#63642, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-GRK2 antibody (Cat#63642). GRK2 expression in wild-type (WT) and GRK2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-GRK2 antibody (Cat#63642, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of GRK2 expression in HepG2 cells using GRK2 antibody (Cat#63642, 1:2,000). Green, isotype control; red, GRK2. |
 | Immunocytochemical staining of HepG2 cells with GRK2 antibody (Cat#63642, 1:1,000). Nuclei were stained blue with DAPI; GRK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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