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Reactivity: | Human, Mouse, Rat |
Applications: | WB, FC |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Monoclonal antibody |
Gene Name: | G protein-coupled receptor kinase 6 |
Gene Symbol: | GRK6 |
Synonyms: | GPRK6 |
Gene ID: | 2870 |
UniProt ID: | P43250 |
Clone ID: | 24GB4870 |
Immunogen: | A synthesized peptide derived from human GRK6 |
Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
Purification Method: | Affinity purified |
Concentration: | Lot dependent |
Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. Several transcript variants encoding different isoforms have been described for this gene.
Images
![]() | Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
![]() | Western blotting analysis using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283). G protein-coupled receptor kinase 6 expression in wild type (WT) and G protein-coupled receptor kinase 6 (GRK6) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
![]() | Flow cytometric analysis of G protein-coupled receptor kinase 6 expression in HepG2 cells using anti-G protein-coupled receptor kinase 6 antibody (Cat#63283, 1:2,000). Green, isotype control; red, G protein-coupled receptor kinase 6. |
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