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| Reactivity: | Human |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | glycophorin C (Gerbich blood group) |
| Gene Symbol: | GYPC |
| Synonyms: | GE; GPC; GPD; GYPD; CD236; PAS-2; CD236R; PAS-2' |
| Gene ID: | 2995 |
| UniProt ID: | P04921 |
| Clone ID: | 24GB5960 |
| Immunogen: | A synthesized peptide derived from human Glycophorin C |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, but plays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations have been described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duch antigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin C protein has very little homology with glycophorins A and B. Alternate splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-glycophorin C antibody (Cat#61143). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-glycophorin C antibody (Cat#61143, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-glycophorin C antibody (Cat#61143). Glycophorin C expression in wild-type (WT) and GYPC shRNA knockdown (KD) HAP-1 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-glycophorin C antibody (Cat#61143, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). GYPC, glycophorin C (Gerbich blood group). |
 | Immunocytochemical staining of HAP1 cells with anti-glycophorin C antibody (Cat#61143, 1:1,000). Nuclei were stained blue with DAPI; Glycophorin C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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