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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | histidyl-tRNA synthetase 1 |
| Gene Symbol: | HARS1 |
| Synonyms: | HRS; HARS; CMT2W; USH3B |
| Gene ID: | 3035 |
| UniProt ID: | P12081 |
| Clone ID: | 24GB6755 |
| Immunogen: | Recombinant protein of human HARS |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-HARS1 antibody (Cat#63655). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HARS1 antibody (Cat#63655, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-HARS1 antibody (Cat#63655). HARS1 expression in wild-type (WT) and HARS1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HARS1 antibody (Cat#63655, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of HARS1 expression in HepG2 cells using anti-HARS1 antibody (Cat#63655, 1:2,000). Green, isotype control; red, HARS1. |
 | Immunocytochemical staining of HepG2 cells with anti-HARS1 antibody (Cat#63655, 1:1,000). Nuclei were stained blue with DAPI; HARS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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