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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | hypoxanthine phosphoribosyltransferase 1 |
| Gene Symbol: | HPRT1 |
| Synonyms: | HPRT; HGPRT |
| Gene ID: | 3251 |
| UniProt ID: | P00492 |
| Clone ID: | 24GB6185 |
| Immunogen: | A synthesized peptide derived from human HPRT |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
Images
 | Western blotting analysis using anti-pancreatic proteinase 1 antibody (Cat#61202). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pancreatic proteinase 1 antibody (Cat#61202, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-pancreatic proteinase 1 antibody (Cat#61202). Pancreatic proteinase 1 expression in wild-type (WT) and pancreatic proteinase 1 (HPRT1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-pancreatic proteinase 1 antibody (Cat#61202, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of pancreatic proteinase 1 expression in HepG2 cells using pancreatic proteinase 1 antibody (Cat#61202, 1:2,000). Green, isotype control; red, pancreatic proteinase 1. |
 | Immunocytochemical staining of HepG2 cells with anti-pancreatic proteinase 1 antibody (Cat#61202, 1:1,000). Nuclei were stained blue with DAPI; Pancreatic proteinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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