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| Reactivity: | Human, Mouse |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | microtubule affinity regulating kinase 2 |
| Gene Symbol: | MARK2 |
| Synonyms: | EMK1; EMK-1; PAR-1; Par1b; Par-1b |
| Gene ID: | 2011 |
| UniProt ID: | Q7KZI7 |
| Clone ID: | 24GB675 |
| Immunogen: | A synthesized peptide derived from human MARK2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene MARK2 encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-MARK2 antibody (Cat#62402). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MARK2 antibody (Cat#62402, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-MARK2 antibody (Cat#62402). MARK2 expression in wild type (WT) and MARK2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MARK2 antibody (Cat#62402, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of MARK2 expression in HT-1080 cells using MARK2 antibody (Cat#62402, 1:2,000). Green, isotype control; red, MARK2. |
 | Immunocytochemical staining of HT-1080 cells with anti-MARK2 antibody (Cat#62402, 1:1,000). Nuclei were stained blue with DAPI; MARK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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