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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Epidermal growth factor receptor |
| Gene Symbol: | EGFR |
| Synonyms: | ERBB; ERRP; HER1; mENA; ERBB1; NNCIS; PIG61; NISBD2 |
| Gene ID: | 1956 |
| UniProt ID: | P00533 |
| Clone ID: | 25GB3430 |
| Immunogen: | A synthesized peptide derived from human Phospho-EGFR (Tyr1068) |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein EGFRencoded by the gene EGFR is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Images
 | Western blotting analysis using anti-phospho-EGFR (Tyr1068) antibody (Cat#61720). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-EGFR (Tyr1068) antibody (Cat#61720, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-phospho-EGFR (Tyr1068) antibody (Cat#61720). Phospho-EGFR (Tyr1068) expression in wild-type (WT) and EGFR shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-EGFR (Tyr1068) antibody (Cat#61720, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Phospho-EGFR (Tyr1068) expression in HepG2 cells using anti-Phospho-EGFR (Tyr1068) antibody (Cat#61720, 1:2,000). Green, isotype control; red, Phospho-EGFR (Tyr1068). |
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