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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | eukaryotic translation initiation factor 2 subunit alpha |
| Gene Symbol: | EIF2S1 |
| Synonyms: | EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha |
| Gene ID: | 1965 |
| UniProt ID: | P05198 |
| Clone ID: | 24GB3905 |
| Immunogen: | A synthesized peptide derived from human Phospho-eIF2 alpha (Ser51) |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).
Images
 | Western blotting analysis using anti-phospho-eIF2 alpha (Ser51) antibody (Cat#63068). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-eIF2 alpha (Ser51) antibody (Cat#63068, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-phospho-eIF2 alpha (Ser51) antibody (Cat#63068). Phospho-eIF2 alpha (Ser51) expression in wild-type (WT) and EIF2S1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-eIF2 alpha (Ser51) antibody (Cat#63068, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). EIF2S1, eukaryotic translation initiation factor 2 subunit alpha. |
 | Flow cytometric analysis of phospho-eIF2 alpha (Ser51) expression in HT-1080 cells using anti-phospho-eIF2 alpha (Ser51) antibody (Cat#63068, 1:2,000). Green, isotype control; red, phospho-eIF2 alpha (Ser51). |
 | Immunocytochemical staining of HT-1080 cells with anti-Phospho-eIF2 alpha (Ser51) antibody (Cat#63068, 1:1,000). Nuclei were stained blue with DAPI; Phospho-eIF2 alpha (Ser51) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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